Supplemental Information Flexible Architecture of IP 3 R 1 by Cryo
نویسندگان
چکیده
Figure S1 (related to Figure 1). Biochemical and functional characterization of the purified IP 3 R1. (A) SDS-PAGE of the purified IP 3 R1 protein: Coomassie blue stained SDS-PAGE gel (left panel) and immunoblot with an IP 3 R1-specific antibody (right panel): lane 1 – molecular weight standards (kDa); lane 2 – ER microsomal membranes; lane 3 – the purified IP 3 R1. The band corresponding to the IP 3 R1 is indicated with an arrowhead. Both, SDS-PAGE and immunoblotting analysis show no detectable degradation of the purified IP 3 R1. (B) Changes in Fura-2 340/380 nm fluorescence ratio as a function of 2 μM IP 3-induced Ca 2+ efflux from empty liposomes (yellow) and IP 3 R-liposomes (purple). Error bars indicate s.e.m (n=5).
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